Part:BBa_K1740000:Design

Dronpa 145N ORF (E. coli optimized)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Non

Source

synthesis DNA

References

[1] Xin X. Zhou, Hokyung K. Chung, Amy J. Lam, and Michael Z. Lin ;Optical control of protein activity by fluorescent protein domains

[2] Jared E. Toettcher, Delquin Gong, Wendell A. Lim, and Orion D. Weiner ;Light control of plasma membrane recruitment using the Phy-PIF system

[3] Hideaki Mizuno, Tapas Kumar Mal, Markus Walchli, Akihiro Kikuchi, Takashi Fukano, Ryoko Ando, Jeyaraman Jeyakanthan, Junichiro Taka, Yoshitsugu Shiro, Mitsuhiko Ikura, and Atsushi Miyawaki ;Light-dependent regulation of structural flexibility in a photochromic fluorescent protein

[4] Martin Andresen, Andre C Stiel, Jonas Folling, Dirk Wenzel, Andreas Schonle, Alexander Egner, Christian eggeling, Stefan W Hell and Stefan Jakobs; Photoswitchable fluorescent proteins enable monochoromatic multilabel imaging and dual color fluorescence nanoscopy

[5] Xin x. Zhou and Michael Z. Lin; Photoswitchable Fluorescent Proteins: TenYears of Colorful Chemistry and Exciting Applications

[6] E. N. Baker and Z. Dauter; Structural basis for the influence of a single mutation K145N on the oligomerization and photoswitching rate of Dronpa